Vascular endothelial growth factor-bound stents: application of in situ capture technology of circulating endothelial progenitor cells in porcine coronary model.

2016 Fiscal Year Final Research Report

• Project/Area Number: 26461105
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Cardiovascular medicine
• Research Institution: Kanazawa University
• Principal Investigator: Yamagishi Masakazu 金沢大学, 医学系, 教授 (70393238)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 血管内皮前駆細胞 / 冠動脈ステント / 新生内膜

Outline of Final Research Achievements

We developed VEGF-bound stents that may enable selective capture of EPCs followed by rapid endothelialization. Metallic stents were first coated with poly-(ethylene-co-vinyl alcohol), and then chemically bound with either VEGF. These stents were placed in porcine coronary arteries. Stent surface was evaluated by immunohistochemistry and by scanning electron microscope. VEGF-bound stents, small populations of KDR positive cells adhered to the stent struts. After long-term stenting, struts were fully covered with newly regenerated tissue. SEM images showed that the uniform tissue formed on struts was morphologically similar to native endothelium and was continuously connected with adjacent native endothelium. VEGF-bound stents provide highly selective capture of EPCs, followed by rapid formation of intact endothelium tissue at an early period of stenting. These results suggest that VEGF-bound stents could represent a promising therapeutic option for cardiovascular stenting.

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