Development of New Less Invasive Preclinical Conscious Mini-pig Model of Ischemia-reperfusion Injury and Mitochondoria Targeting Nano-therapy

2016 Fiscal Year Final Research Report

• Project/Area Number: 26461131
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Cardiovascular medicine
• Research Institution: Kyushu University
• Principal Investigator: Nakano Kaku 九州大学, 循環器病未来医療研究センター, 准教授 (30420607)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 分子心臓学 / ナノメディシン

Outline of Final Research Achievements

Early reperfusion therapy is a standard strategy to reduce MI size. However, reperfusion induces ischemia-reperfusion (IR) injury, which reduces the therapeutic effects of reperfusion therapy. Mini-pigs were surgically implanted with a pneumatic cuff occluder at the left circumflex coronary artery. The LCx was occluded for 60 minutes, followed by 24 hours of reperfusion under conscious conditions. Intravenous administration of pitavastatin-NP containing of pitavastatin 5 minutes before reperfusion significantly reduced infarct size; by contrast, pitavastatin alone (8 mg/body) showed no therapeutic effects. Cardiac magnetic resonance imaging performed 4 weeks after IR injury revealed that pitavastatin-NP reduced the extent of left ventricle remodeling. NP-mediated delivery of pitavastatin to IR-injured myocardium exerts cardioprotective effects on IR injury without apparent adverse side effects in a preclinical conscious pig model.

Free Research Field

循環器内科学