Configuration of bacteriological evidence of causative pathogens in patients with aspiration pneumonia using the bacterial floral analysis of 16S ribosomal RNA gene

2016 Fiscal Year Final Research Report

• Project/Area Number: 26461172
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Respiratory organ internal medicine
• Research Institution: University of Occupational and Environmental Health, Japan
• Principal Investigator: Mukae Hiroshi 産業医科大学, 医学部, 非常勤医師 (80253821)
• Research Collaborator: AKATA Kentaro ; KAWANAMI Toshinori ; NOGUCHI Shingo ; NAITO Keisuke ; HATA Ryosuke ; NISHIDA Chinatsu ; YAMASAKI Kei ; KIDO Takashi ; YATERA Kazuhiro
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 誤嚥性肺炎 / 口腔レンサ球菌 / 嫌気性菌 / 細菌叢解析 / 16S rRNA / 口腔内衛生状態

Outline of Final Research Achievements

We evaluated causative bacterial species by the clone library method using 16S rRNA gene sequencing analysis of bronchoalveolar lavage fluid in pneumonia patients (n=177) with or without aspiration risks. Oral streptococci were significantly detected in patients with aspiration risks (n=83) compared with those without, and factors such as poor ECOG performance status, past history of pneumonia within one year were associated with a detection of oral streptococci. These results suggest that oral streptococci are more important as causative pathogens of pneumonia in patients with aspiration pneumonia than previously believed. In addition, studies evaluated the relationship between oral hygiene status and detected bacterial species showed that poor oral hygiene status was significantly related to higher detection of anaerobic bacteria, and therefore oral hygiene status might be important factor for detecting anaerobic bacteria in the lower respiratory tract infection.

Free Research Field

呼吸器感染症