steroid resistance of upper and lower airways

2016 Fiscal Year Final Research Report

• Project/Area Number: 26461197
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Respiratory organ internal medicine
• Research Institution: Keio University
• Principal Investigator: Fukunaga Koichi 慶應義塾大学, 医学部(信濃町), 講師 (60327517)
• Co-Investigator(Renkei-kenkyūsha): ARITA Makoto 慶應義塾大学, 薬学部, 教授 (80292952) ; MORO Kazuyo 国立研究開発法人理化学研究所, 総合生命医科学研究センター, チームリーダー (90468489)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: ステロイド抵抗性 / 自然免疫リンパ球 / 好酸球

Outline of Final Research Achievements

In conducting our research regarding steroid resistance of upper and lower airways, we collected　eosinophils and Group 2 innate lymphoid cells (ILC 2s) from surgical specimens of chronic eosinophilic sinusitis and peripheral blood of severe asthmatic patients and examined them. First, we established a method to collect each cell stably from the specimen and blood. Especially, ILC2s with small number in blood was examined using single cell analysis method which can analyze from one cell. As a result, the difference between the healthy subjects and the patient's phenotype (proliferative ability and cytokine production capacity) has become clear. In addition, eosinophils were also examined using comprehensive analysis and revealed differences in productivity of enzymes and mediators as compared to healthy subjects. We are currently investigating the resistance of steroids of these cells and will continue to conduct current research.

Free Research Field

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