Mesenchymal stem cells cultured in serum-free medium ameliorate experimental renal fibrosis.

2016 Fiscal Year Final Research Report

• Project/Area Number: 26461229
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Kidney internal medicine
• Research Institution: Hiroshima University
• Principal Investigator: MASAKI TAKAO 広島大学, 病院(医), 教授 (30397913)
• Research Collaborator: KATO YUKIO 広島大学, 名誉教授 (10112062)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 再生医療 / 間葉系幹細胞 / 線維化 / 抗炎症作用

Outline of Final Research Achievements

The Mechanism underlying the anti-inflammatory effect of mesenchymal stem cells (MSCs) has been gradually elucidated. However, the effect of MSCs cultured in serum-free medium has not been clarified. We investigated the difference between MSCs cultured under 10 % fetal bovine serum (FBS) containing medium and serum-free medium of the therapeutic effects in a rat model of renal fibrosis. After 4 days of unilateral ureteral obstruction (UUO) operation, we injected rat MSCs cultured with 10% FBS containing DMEM (10%MSCs) or serum-free medium (SF-MSCs), or PBS only (PBS) through the rat tail vein. As a results, transplantation of SF-MSCs potently inhibited the infiltration of inflammatory cells and the progression of fibrosis compared with 10%MSCs.

Free Research Field

腎臓病学