2016 Fiscal Year Final Research Report
Can autophagy attenuate vascular calcification?
Grant-in-Aid for Scientific Research (C)
Kidney internal medicine
|Project Period (FY)
2014-04-01 – 2017-03-31
|オートファジー / 腎不全 / 血管石灰化 / Calciprotein particle
|Outline of Final Research Achievements
Calciprotein particle (CPP) efficiently induced autophagy in human endothelial smooth muscle cells (SMC). Calcification induced by high phosphorus or CPP was attenuated in Atg7-knockdown SMCs. To explore the mechanism of attenuated calcification, we examined gene expression related with osteoblastic transformation of SMC, However, we were not able to find the change of gene expression in Atg7 knockdown SMC. Nextly, we dissected mouse aorta and incubated the piece of aorta ex vivo. In high phosphorus, calcification of media was observed, on the other hand, calcification of tunica adventitia was observed in CPP-added medium. We are going to start ex vivo culture of aorta derived from smooth muscle cell-specific Atg7 deleted mice.
|Free Research Field