Evaluation of 3-factor model (3F) of renal urate transport that may avoid less side-effect of druf-induced hyperuricemia.

2016 Fiscal Year Final Research Report

• Project/Area Number: 26461258
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Kidney internal medicine
• Research Institution: Chiba University (2015-2016); Dokkyo Medical University (2014)
• Principal Investigator: Anzai Naohiko 千葉大学, 大学院医学研究院, 教授 (70276054)
• Co-Investigator(Renkei-kenkyūsha): SATO Motoyasu 獨協医科大学, 医学部, 助教 (20418891) ; OHBAYASHI Tetsuya 鳥取大学, 生命機能研究支援センター, 准教授 (80348804)
• Research Collaborator: HAYASHI Keitaro ; MORITA Asuka ; NOHARA Masakatsu ; WAKASHIN Hidefumi ; TSUCHIYA Go ; HORI Takayuki ; KANO Yuji ; ONIZAWA Nobuyuki ; OTSUKA Yusuke ; OHNO Yuta ; Wempe Michael F. ; ENDOU Hitoshi ; KIMURA Toru ; TAMAI Ikumi
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 尿酸 / 高尿酸血症 / 膜輸送 / トランスポーター / 創薬

Outline of Final Research Achievements

This study was aimed to investigate the effectiveness of a novel concept "3-factor model (3F)" and to make an alternative possibility for the development of new drugs that have less side effects regarding drug-induced hyperuricemia. For developing appropriate materials for evaluating this concept that may express triple urate transporters such as URAT1, URATv1, and NPT4, we performed a collaborative study with Dr. Tetsuya Ohbayashi who is a specialist of a mammalian artificial chromosome vector that can be used for introducing multiple genes and succeded to express several kidney-derived genes using his system onto our mouse-derived renal tubular cell named S2. We will use this protocol for further analysis. In addition, we could raise a new animal model that can be used for analyzing the concept of 3F by using pyrazinamide-loading method for creating renal-underexcretion hyperuricemia model.

Free Research Field

薬理学、生理学