2016 Fiscal Year Final Research Report
A role of kidney intercalated cells type B in regulatory transport of water and electrolytes
| Project/Area Number |
26461259
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Kidney internal medicine
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| Research Institution | Kitasato University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | Ca感知受容体 / 集合管間在細胞 / 酸塩基バランス / 低リン食 |
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| Outline of Final Research Achievements |
By using the highly sensitive ISH technique and a double-staining IHC technique, we examined the axial distribution and expression of the calcium-sensing receptor (CaSR) along the nephron in mice treated for 6 days with water containing acid (NH4Cl) or alkali (NaHCO3)-containing diets. CaSR was localized in the cortical and medullary thick ascending limb of Henle's loop, macula densa, distal convoluted tubule, and cortical collecting duct (CCD). In the CCD, CaSR was co-localized with an anion exchanger type 4, a marker of the basolateral membrane of type-B intercalated cell (IC-B). CaSR expression levels in IC-B significantly decreased when mice were fed NH4Cl and increased when animals were given NaHCO3. Surprisingly, single infusion (ip) of neomycin, an agonist of CaSR, significantly increased urinary Ca excretion without further increasing the hourly urine volume. Moreover, urine pH was never decreased when urinary excretion of Ca was increased in phosphate-deprived mice.
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| Free Research Field |
腎生理学
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