Expression profiling of inclusion body myositis by massive parallel sequencing

2016 Fiscal Year Final Research Report

• Project/Area Number: 26461265
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Neurology
• Research Institution: International University of Health and Welfare (2015-2016); The University of Tokyo (2014)
• Principal Investigator: Goto Jun 国際医療福祉大学, 大学病院, 教授 (10211252)
• Co-Investigator(Renkei-kenkyūsha): ISHIURA Hiroyuki 東京大学, 医学部附属病院, 助教 (40632849)
• Research Collaborator: IKENAGA Chiseko 東京大学, 大学院医学系研究科脳神経医学専攻
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 炎症性筋疾患 / 封入体筋炎 / 縁取り空胞変性 / RNA-seq / 発現プロファイリング / レーザーマイクロダイセクション

Outline of Final Research Achievements

The aim of this study was to elucidate pathophysiology of inclusion body myositis by comprehensive expression profiling. Seventy-nine samples (11 controls, 11 PM, 47 IBM and 10 non-PM, non-IBM MHC-1 complex positive cases) were subjected to RNA-seq analysis. Seventy-one cases, of which RIN were more than 5, were statistically analyzed. Cluster analysis suggested that there was correlation between morphological classification and expression profile cluster. In IBM expression of cell adhesion molecules, inflammatory system, autophagy-lysosome system, and splicesome system were up-regulated. Expression of oxidative phosphorylation, citric cycle and electron transport genes was down-regilated.

Free Research Field

神経内科学