Research about S-nitrosylation of proteins related to pathogenesis of Parkinson's disease

2017 Fiscal Year Final Research Report

• Project/Area Number: 26461277
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Neurology
• Research Institution: Osaka University (2017); Nara Medical University (2014-2016)
• Principal Investigator: Ozawa Kentaro 大阪大学, 医学部附属病院, 医員 (80507393)
• Co-Investigator(Kenkyū-buntansha): 吉栖 正典 奈良県立医科大学, 医学部, 教授 (60294667)
• Project Period (FY): 2014-04-01 – 2018-03-31
• Keywords: nitric oxide / s-nitrosylation / Parkinson's disease

Outline of Final Research Achievements

I have revealed parkin, a protein related to pathogenesis of Parkinson's disease, is S-nitrosylated and its activity is regulated by S-nitrosylation. To investigate a role of S-nitrosylation of endogenous parkin in degeneration in Parkinson's disease, we generated cell lines which cysteine targeted by S-nitrosylation was converted to another amino acid. Using them, I revealed S-nitrosylation of endogenous parkin is not involved in degradaiton of mitochondria through autophagy but in cell death by mitochondrial stress. These findings indicated S-nitrosylation of parkin might play a important role in cell death by mitochondrial dysfunction and , at least in part, pathogenesis of Parkinson's disease.

Free Research Field

神経薬理