Effects of microRNA on the risk of sporadic Parkinson disease associated with NACP-Rep1 polymorphic site

2016 Fiscal Year Final Research Report

• Project/Area Number: 26461283
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Neurology
• Research Institution: Mukogawa Women's University
• Principal Investigator: Mizuno Hideya 武庫川女子大学, 薬学部, 准教授 (90322578)
• Co-Investigator(Renkei-kenkyūsha): NAGAI Yoshitaka 大阪大学, 医学系研究科, 教授 (60335354)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 神経変性疾患 / パーキンソン病 / マイクロRNA / SNP / αシヌクレイン

Outline of Final Research Achievements

Poly (ADP Ribose) Polymerase-1 (PARP1) binds to the NACP-Rep1 polymorphic site upstream of α-Synuclein (αSyn) gene and negatively regulates αSyn expression. When the construction of the plasmid carrying the PARP1 and miRNA-124 (miR-124) were cotransfected into cultured cells, miR-124 significantly reduced PARP1 expression. In order to specify the inhibitory mechanism, we mutated the predicted target sequence of miR-124 on the PARP1 mRNA 3’ untranslated region in the plasmid by substitution or deletion of the nucleotides. The mutation partly eliminated the inhibition of PARP1 expression by miR-124. These results suggest that miR-124 inhibits PARP1 expression by binding not only directly to PARP1 mRNA 3’untranslated region, but also to another target mRNA.

Free Research Field

細胞生物学