Anti-endothelial cell autoantibodies associated with cerebral small vessel disease

2016 Fiscal Year Final Research Report

• Project/Area Number: 26461290
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Neurology
• Research Institution: Gifu University
• Principal Investigator: Kimura Akio 岐阜大学, 大学院医学系研究科, 准教授 (00362161)
• Research Collaborator: YOSHIKURA Nobuaki 岐阜大学, 医学部附属病院, 助教 ; KOUMURA Akihiro 岐阜市民病院, 神経内科, 部長 ; TAKEMURA Masao 岐阜大学, 医学部附属病院, 検査技師 ; INUZUKA Takashi 岐阜大学, 大学院医学系研究科, 教授
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 抗血管内皮抗体 / 脳小血管病変 / Annexin A2 / Amyloid β / Hexokinase 1

Outline of Final Research Achievements

We detected the anti-endothelial cell autoantibodies in sera and cerebrospinal fluid (CSF) from patients with neurological diseases by 2-dimensional immunoblotting or immunoprecipitation using primary cultured human brain microvascular endothelial cells as the antigen source. Subsequently, we analyzed the target antigens of these autoantibodies by liquid chromatography-tandem mass spectrometry. We also analyzed the association between the titer of identified anti-endothelial cell autoantibodies and the severity of cerebral small vessel disease. We detected the autoantibody against hexokinase 1, which is a mitochondrial outer membrane protein and play a role for regulation of energy production and apoptosis, in sera from the patient with severe small vessel disease. We also found that the serum anti-Annexin A2 autoantibodies and CSF anti-amyloid β autoantibodies are associated with the severity of cerebral small vessel disease in patients with Alzheimer's disease.

Free Research Field

医歯薬学