2016 Fiscal Year Final Research Report
Ischemic stroke therapy using quality and quantity-controlled culture system enriched in endothelial progenitor cells
| Project/Area Number |
26461320
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Neurology
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| Research Institution | Tokai University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | 単核球培養 / 血管内皮前駆細胞 / 脳梗塞 |
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| Outline of Final Research Achievements |
Quality and quantity-controlled culture system enriched in endothelial progenitor cells (EPCs) from unfractionated mononuclear cells (QQ-MNCs), which we developed, have a potential to activate anti-inflammatory cytokines and expanding vasculogenic EPCs. QQMNCs injected to mice at 1-day after occlusion of middle cerebral artery (MCA) reduced the rate of ischemic stroke volume compared with PBS. The immunohistochemistry showed the increase of positive cells reacted with VEGF and IL-10 after 7 and 21-days in QQ-MNCs-treated mice. These findings reveal that QQ-MNCs might promote repair and regeneration of neurovascular units after acute focal ischemic stroke.
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| Free Research Field |
神経内科学
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