2017 Fiscal Year Final Research Report
Patient-derived iPS cell for the elucidation of diabetic pathogenesis.
| Project/Area Number |
26461330
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Metabolomics
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| Research Institution | Kyoto University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2018-03-31
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| Keywords | 糖尿病 / iPS細胞 / ミトコンドリア / 脂肪 / seipin |
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| Outline of Final Research Achievements |
We generated several series of diabetic patient-derived iPS cells.Mitochondrial diabetic patient-derived iPS cells showed bimodal levels of mutation(A3243) frequency among each clone. Those mutation-rich and -free iPS cells could differentiate into insulin-producing cells. Congenital generalized lipodystrophy patients had seipin gene mutation. Lipodystrophy iPS cells had scarce lipid droplets, reflecting patients' phenotype. We found abnormal perilipin localization from ER to cytosol in these iPS cells. The finding indicates that the causative gene seipin has a role relating the localization of lipid droplets forming protein.
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| Free Research Field |
糖尿病
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