2016 Fiscal Year Final Research Report
Finding the way to control ghrelin activity for the obesity cure
| Project/Area Number |
26461331
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Metabolomics
|
|---|
| Research Institution | Wakayama Medical University (2015-2016)
Kyoto University (2014) |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2014-04-01 – 2017-03-31
|
| Keywords | グレリン / GPCR / IL-1 |
|---|
| Outline of Final Research Achievements |
We determined the comprehensive mRNA expression profiles in the ghrelin-producing cell line, MGN3-1 cells. By examining the ligands for the highly expressed GPCR, we found that prostaglandin E2 and tryptophan stimulated ghrelin secretion via EP4 receptor and GPR142, respectively. Except for GPCR, we found that IL-1b significantly suppressed ghrelin mRNA expression in MGN3-1 cells, at least partly through NF-kB pathway. We found that ghrelin-producing cells highly incorporated long-chain fatty acids. The high incorporation of long-chain fatty acids to the cells, which was provided by high levels of Acsl1, contributed to the efficient acylation of ghrelin.
|
| Free Research Field |
内分泌代謝学
|
