2016 Fiscal Year Final Research Report
Development for pancreatic beta cell imaging method by using a fluorescent probe
| Project/Area Number |
26461334
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Metabolomics
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| Research Institution | Kyoto University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | 膵島イメージング / 蛍光イメージング |
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| Outline of Final Research Achievements |
In this study, I start to develop the imaging method for measuring a pancreatic beta cell mass by using fluorescent probe. A fluorescent probe with exendin frame was prepared. The probe targeted a glucagon like-peptide 1 receptor (GLP-1R) which highly expressed in pancreatic beta cells. To evaluate the probe properties, binding affinity study and binding study for cells, and immunostaining study of pancreatic slices were performed. The specific binding for GLP-1R was shown by binding affinity study. And the binding for cell with GLP-1R was shown by fluorescent image analysis and flow cytometry analysis.
These studies showed that the fluorescent probe with exendin has a possibility for imaging the pancreatic beta cell and analyzing the mass.
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| Free Research Field |
糖尿病
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