2016 Fiscal Year Final Research Report
Roles of AFF4, a member of the AF4 family of transcription factors, in FGF21 resistance
| Project/Area Number |
26461365
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Metabolomics
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| Research Institution | Wakayama Medical University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
HISAOKA TOMOKO 和歌山県立医科大学, 医学部, 助教 (00398463)
FURUTA HIROTO 和歌山県立医科大学, 医学部, 准教授 (90238684)
KITAMURA TOSHIO 東京大学, 医学部, 教授 (20282527)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | AFF4 / 転写因子 / FGF21 / メタボリック症候群 / 摂食調節 / 視床下部 |
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| Outline of Final Research Achievements |
The expression of AFF4 was observed in NPY/AgRP neurons in the hypothalamus during fasting. Up-regulation of NPY and AgRP observed in the fasted states of WT mice was attenuated in heterozygous AFF4-deficient mice. These results suggest that AFF4 regulates the expression of NPY and AgRP in the hypothalamus during fasting. Next, we investigated the roles of FGF21 on the expression of AFF4 in mice. The expression levels of AFF4 are not changed by the treatment with FGF21 in the hypothalamus, liver, and adipose tissue. During fasting, the body temperature is reduced in WT mice. In heterozygous AFF4-deficient mice, the body temperature in the fasted state was higher than WT mice with more heat production. Furthermore, locomotor activity in heterozygous AFF4-deficient mice was lower compared to WT mice in both fed and fasted states.
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| Free Research Field |
代謝・内分泌
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