2017 Fiscal Year Final Research Report
Analysis of protein regulating brain-specific aromatase gene expression through proteomics
| Project/Area Number |
26461389
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Endocrinology
|
|---|
| Research Institution | Fukuoka University |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2014-04-01 – 2018-03-31
|
| Keywords | ステロイドホルモン / 神経細胞 / 性分化 / 転写制御 |
|---|
| Outline of Final Research Achievements |
In order to clarify the spatiotemporal-specific expression mechanism of aromatase involved in sexual differentiation of the brain, I performed functional analysis of Deaf1, a transcription factor responsible for the regulation of the aromatase expression in the brain. The diencephalic region was excised from the fetal mouse brain, and a nuclear extract was prepared. To obtain a sample containing protein interacting with Deaf1, immunoprecipitation reaction was carried out using an antibody recognizing endogenous Deaf1 protein. The obtained sample was subjected to SDS-PAGE, and protein band was excised. LC-MS / MS analysis of the sample revealed that Transcriptional Intermediary Factor-1β (TIF-1β) is as one of the molecule interacting with the Deaf1 protein.
|
| Free Research Field |
生化学
|
