2016 Fiscal Year Final Research Report
Analysis of functions of a receptor tyrosine kinase selectively expressed in plasmacytoid dendritic cells.
| Project/Area Number |
26461465
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Collagenous pathology/Allergology
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| Research Institution | Kagawa University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | 形質細胞様樹状細胞 / Ⅰ型インターフェロン / 全身性エリテマトーデス |
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| Outline of Final Research Achievements |
We have demonstrated that an Ephrin receptor tyrosine kinase is selectively expressed in mouse plasmacytoid dendritic cells (pDCs) by use of a specific monoclonal antibody. When we incubate pDCs in vitro in the presence or absence of this monoclonal antibody, then stimulate with Toll-like receptor 7(TLR7) or TLR9 ligands, we have found that pDC produce comparable levels of cytokines including interferon-alpha. We have also found that pDCs normally developed in Flt3 ligand-induced bone marrow derived dendritic cells cultured in the presence of this monoclonal antibody. In order to analyze the function of the Ephrin receptor tyrosine kinase in mouse plasmacytoid dendritic cells, we have established two lines of knockout mouse. We are planning to analyze the phenotype of pDCs in these knockout mice.
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| Free Research Field |
免疫学
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