Analysis of cell death of sputum neutrophils aiming at development of a diagnostic method for new pneumonia

2016 Fiscal Year Final Research Report

• Project/Area Number: 26461502
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Infectious disease medicine
• Research Institution: Osaka University
• Principal Investigator: YOSHIDA HISAO 大阪大学, 医学部附属病院, 助教 (80598574)
• Research Collaborator: TAKEGAWA RYOSUKE 大阪大学, 医学部附属病院, 医員 ; HAMAGUCHI SHIGETO ニューヨーク市立大学, 研究員 ; YAMAMOTO NORIHISA 大阪大学, 医学部附属病院, 医員
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 肺炎 / 好中球 / グラム染色 / NETs / hyper spectral imaging

Outline of Final Research Achievements

The diagnosis of pneumonia is still difficult now. We observed Gram stained slides of sputum collected from patients with pneumonia for observation with an optical microscope using a dedicated camera that can analyze spectral data in detail. With this camera we were able to obtain data dividing the image in the neutrophils every 5 nm in the wavelength range of 400 to 1000 nm of light. By analyzing the obtained data with dedicated software, it is possible to distinguish differences in color tone that can not be discerned by human eyes. It was shown that the difference in color can be distinguished and classified as the difference in activation of neutrophils.

Free Research Field

感染症治療