2016 Fiscal Year Final Research Report
Development of useful method in differential diagnosis of Creutzfeldt-Jakob diseases
| Project/Area Number |
26461507
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Infectious disease medicine
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| Research Institution | University of Miyazaki (2016)
Nagasaki University (2014-2015) |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
ATARASHI Ryuichiro 宮崎大学, 医学部, 教授 (90452846)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | パネルRT-QUIC法 / 孤発性CJD |
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| Outline of Final Research Achievements |
Sporadic Creutzfeldt-Jakob disease (sCJD), a majority of human prion diseases, is classified in different subtypes which contribute to the occurrence of distinct clinical-pathological phenotypes. In this study, we sought to determine whether a modification of real-time quaking-induced conversion (RT-QUIC) assay, a sensitive diagnostic test for prion diseases, allows for distinguish between subtypes of sCJD. We constructed a several different types of recombinant prion protein (recPrP) mutant as a substrate for RT-QUIC reaction. We compared the prion detective sensitivities of RT-QUIC reaction with mutant recPrPs. RT-QUICs were performed with brain homogenates from several types of prion disease patients or infected mice. As a result, there were slightly differences in RT-QUIC signals between specimens by using mutant recPrP as substrate.
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| Free Research Field |
分子生物学
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