2016 Fiscal Year Final Research Report
A study of chemokine receptor dynamics and its inhibitor(s) in HIV infection
| Project/Area Number |
26461510
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Infectious disease medicine
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| Research Institution | Kumamoto University |
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Principal Investigator |
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| Research Collaborator |
Maeda Kenji
Iwami Shingo
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | HIV感染症 / CCR5 / 侵入阻害薬 |
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| Outline of Final Research Achievements |
We constructed wild type CCR5(CCR5-WT) and mutant CCR5(CCR5-MT), which loses susceptibility against HIV, coexpressing cells and tested HIV infectivity using these cells.10% and 50% levels of CCR5-MT expression reduced the susceptibility against HIV by 50% and almost 100% compared with only CCR5-WT espressing cells, respectively. This result strongly suggested that multiple CCR5 are required for the HIV entry step. Next we developed a mathematical model based on the correlation between the ratio of CCR5-MT expression and HIV susceptibility, and estimated the numbers of CCR5 required for the HIV infection. As a result we quantitatively estimated 6-8 CCR5s are required for gp120 trimer-CCR5 interaction. These data revealed a part of the entry step of HIV infection.
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| Free Research Field |
感染症
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