Investigation of detection method for Vancomycin intermediated-resistant Staphylococcus aureus

2016 Fiscal Year Final Research Report

• Project/Area Number: 26461513
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Infectious disease medicine
• Research Institution: Kitasato University
• Principal Investigator: Hanaki Hideaki 北里大学, 北里生命科学研究所, 特任教授 (60286747)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: バンコマイシン / VISA / ヘテロVISA / 検出 / 混合培養

Outline of Final Research Achievements

Antibiotic resistant cells emerge spontaneously at a certain frequency in the bacterial population that is termed as hetero-resistance. One of the most problematic antibiotic resistant bacteria in the clinical setting may be Staphylococcus aureus MRSA that exhibits resistance to structurally diverse multiple antibiotics including vancomycin. Among them, vancomycin intermediate resistant cells (VISA) appear in the population of vancomycin heteroresistant cells (hVISA). Therefore, detection of VISA cells in the hVISA population is difficult due to the fact that VISA appears at the frequency of 0.012% of hVISA. To detect VISA by the MIC method, over 10 times of cells are required compared with that of the conventional MIC determination. To overcome this difficulty, studies are on progress to detect VISA specific proteins by using MALDI-TOF-Mass spectroscopy.

Free Research Field

感染症学　細菌学