2016 Fiscal Year Final Research Report
Elucidation of construction abnormality of brain cytokine network caused by juvenile stress
| Project/Area Number |
26461521
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Pediatrics
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| Research Institution | Teikyo University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2017-03-31
|
| Keywords | 母子分離ストレス / ミクログリア |
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| Outline of Final Research Achievements |
The brain, spleen, liver, and the bone marrow in the mouse gave maternal separation stress for seven days were harvested for this study. As the results showed, the microglia in separation group was the F4/80 negative cell while the microglia cell in control group was F4/80-positive cells. The expression of the microglial specific protein significantly decreased in the brain by the western blotting in separation group. However there were no significant effect in spleen.
Moreover, the cell properties of the microglial cells isolated a pure microglial cells by FACS method. As for the isolated microglial cell, the property parts from FACS analysis for three group populations, and it is suggested that the origin of microglia may change by pre or postnatal stress and it may affect to the adult stage brain immunity.
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| Free Research Field |
神経科学
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