2016 Fiscal Year Final Research Report
Elucidation of peroxisomal disease mechanisms by analyzing novel lipid molecules in the patient's serum and applying them as a biomarker
| Project/Area Number |
26461532
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Pediatrics
|
|---|
| Research Institution | Teikyo University |
|---|
Principal Investigator |
|
|---|
| Research Collaborator |
SHIMOZAWA Nobuyuki
IMANAKA Tsuneo
|
|---|
| Project Period (FY) |
2014-04-01 – 2017-03-31
|
| Keywords | リピドミクス / メタボローム / 極長鎖脂肪酸 / 副腎白質ジストロフィー / ペルオキシソーム / 質量分析 / 先天代謝異常 / リン脂質 |
|---|
| Outline of Final Research Achievements |
The aim of this study was to identify novel lipid molecules whose amounts vary to reflect the status of peroxisomal disease and apply them as a biomarker. We established analysis methods for sphingomyelin and acyl-CoA, as well as a method for comprehensive analysis of glycosphingolipids using mass spectrometry. We analyzed phospholipids from the brain of ABCD1-knockout mice and found an increase in several numbers of species containing very long chain fatty acids. We then determined the molecules’ structures. In addition, we have established an ABCD1-knockout cell line. We have started the identification of a responsive enzyme for the synthesis of phospholipid species containing very long chain fatty acids.
|
| Free Research Field |
脂質生化学
|
