2016 Fiscal Year Final Research Report
Comprehensive pathological analysis of SSPE using exome analysis and patients iPSC-derived neurons.
| Project/Area Number |
26461547
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Pediatrics
|
|---|
| Research Institution | Kyushu University |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2014-04-01 – 2017-03-31
|
| Keywords | SSPE / エキソーム解析 / iPS細胞 |
|---|
| Outline of Final Research Achievements |
As a pathophysiological analysis of the subacute sclerosing panencephalitis, which is intractable disease due to unknown pathophysiology, we planned the genetic analysis for identification of responsible gene and biological analysis for clarifying cellular mechanism using neurons differentiated from iPS cells and genetically modified measles virus.
For the exosome analysis, we analyzed 8 patients of 3 families, which had no consanguineous parentage. We performed analysis assuming the inheritance model as compound heterozygote, but failed to determine the responsible gene. We succeeded in establishing iPS cells from peripheral blood mononuclear cells of 3 patients with subacute sclerosing panencephalitis. There was no morphological difference between neurons differentiated from iPS cells derived from subacute sclerosing panencephalitis patients and those of from healthy control.
|
| Free Research Field |
小児神経学
|
