2016 Fiscal Year Final Research Report
Leukemogenic pathway of TEL-AML1-positive leukemia
| Project/Area Number |
26461587
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Pediatrics
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| Research Institution | Ehime University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | 白血病 / 融合遺伝子 |
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| Outline of Final Research Achievements |
TEL-AML1 is the most common fusion gene in childhood acute lymphoblastic leukemia (ALL). It alone is not enough to cause leukemia, and additional genetic change(s) are necessary. Retroviral vector-mediated insertional mutagenesis was applied on TEL-AML1-expressing hematopoietic progenitor cells to identify the cooperating genetic changes with TEL-AML1 in leukemogenesis. Tie2-positive cells containing immature hematopoietic progenitors were transduced by retroviral vector to induce insertional mutagenesis, and then transplanted into immunodeficient mice. In these mice B220-positive cells were expanded, and tumor formation was observed in some of them. Analysis of retroviral integration site in these tumors showed intra-genic insertion of viral sequences possibly resulting in disruption of gene expression/regulation. Genes located near the integration site may be the target of cooperating genetic changes necessary for leukemogenesis by TEL-AML1.
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| Free Research Field |
小児科学
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