Regulation of the function of endothelial cells by shear stress

2016 Fiscal Year Final Research Report

• Project/Area Number: 26461593
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Pediatrics
• Research Institution: Nara Medical University
• Principal Investigator: shida yasuaki 奈良県立医科大学, 医学部, 助教 (10721566)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 血流 / ずり応力 / 凝固 / 血栓

Outline of Final Research Achievements

1) von Willebrand factor (VWF) plays a dominant role in shear stress mediated thrombus formation. Factor VIII (FVIII) alone did not improve thrombus under high shear. However, in the presence of VWF, FVIII improved thrombus formation, suggesting the importance of FVIII/ VWF interaction in thrombus formation under high shear. 2) We succeeded the culture of endothelial cells (ECs) under shear conditions. ECs demonstrated round shape under static condition and string-like elongated shape under laminar shear condition. Under disturbed flow condition which does not have the clear direction, ECs demonstrated various shapes including round shape to elongated shape which does not have the directions.

Free Research Field

血液