2016 Fiscal Year Final Research Report
Pathogenesis of insufficient adrenocortical response in premature sheep fetus
| Project/Area Number |
26461627
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Embryonic/Neonatal medicine
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| Research Institution | Tohoku University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | 胎児 / 早産児 / 子宮内炎症 / 副腎皮質ホルモン / CRH負荷試験 |
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| Outline of Final Research Achievements |
We made a plan to clarify the effect of intrauterine inflammation on the cortisol secretion and its circulatory response in premature lamb because the fetal endocrine potential to adapt the postnatal environment might have been already deteriorated by the inflammatory stress before birth. In this study, we separated sheep fetuses into two groups: control group (n=6) and inflammation group (n=6), and we could store the plasma samples for 31 times of the corticotrophin releasing hormone (CRH) test. In consequence, we could measured the concentrations of ACTH, cortisol, cortisone, Aldosterone and Dehydroepiandrosterone sulfate (DHEA-S) by the method of steroid profiling analysis using the LC-MS/MS. In the inflammation group than the control, production of cortisol was promoted during the CRH test. These findings suggest the intrauterine inflammation stress might induce the development of transitional zones where 3β-hydroxysteroid dehydrogenase is expressed.
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| Free Research Field |
胎児生理学
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