iTRAQ proteomic analysis of aganglionic colonic mucosa to detect new diagnostic markers in Hirschsprung's disease

2016 Fiscal Year Final Research Report

• Project/Area Number: 26461631
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Embryonic/Neonatal medicine
• Research Institution: Mie University
• Principal Investigator: INOUE Mikihiro 三重大学, 医学部附属病院, 助教 (30422835)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: ヒルシュスプルング病 / プロテオミクス / 診断マーカー

Outline of Final Research Achievements

Rectal mucosal biopsy is essential for the definite diagnosis of Hirschsprung’s disease (HD). However, this examination is sometimes less valuable when applied to neonates. Therefore, a less invasive and more accurate diagnostic approach is required for early screening and diagnosis of HD. In the present study, we used iTRAQ proteomic analysis to identify a novel candidate protein aimed to increase diagnostic accuracy. We prepared resected tissues from 4 patients with HD and 4 subject without HD (non-HD). The colonic mucosa of the aganglionic segment from the patient with HD, colonic mucosa of the ganglionic segment from the same HD patient and colonic mucosa from the non-HD subject were manually microdissected. The proteins were extracted, digested, labeled using iTRAQ reagents, and analyzed using mass spectrometer. We identified and quantitated 197 proteins. Among these proteins, the levels of 17 proteins were significantly up-regulated in the aganglionic HD sample.

Free Research Field

小児外科