Pathogenic microorganism identification in neonatal infectious diseases by a meta-genomic analysis using the next generation sequencer

2016 Fiscal Year Final Research Report

• Project/Area Number: 26461632
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Embryonic/Neonatal medicine
• Research Institution: Kobe University
• Principal Investigator: MORIOKA Ichiro 神戸大学, 医学研究科, 特命教授 (80437467)
• Co-Investigator(Renkei-kenkyūsha): MORI Yasuko 神戸大学, 大学院医学研究科, 教授 (50343257) ; DEGUCHI Masashi 神戸大学, 大学院医学研究科, 特命教授 (50403291)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 次世代シークエンサー / メタゲノミック診断法 / 新生児感染症 / 小児感染症

Outline of Final Research Achievements

The purpose of this study was to establish a meta-genomic analysis using the next generation sequencer for perinatal and neonatal infectious diseases, and to identify pathogenic microorganisms that had been difficult to identify to date. As results, a meta-genomic analysis system using the next generation sequencer was established during the study period and we applied to the neonatal and pediatric patients with infection. We obtained new findings from patient specimens using these techniques, and for the first time, reported the cases to the international journals, such as a newborn with persistent pulmonary hypertension who was detected echovirus 7 from not only the serum, nasal swab and bronchoalveolar lavage, but also the preserved umbilical cord, a pediatric patient due to enterovirus D68 infection that developed interstitial pneumonia, and a newborn with congenital cytomegalovirus infection with retinal arterio-venous anastomosis.

Free Research Field

新生児医学、小児感染症学