2016 Fiscal Year Final Research Report
Preventative agents in mouse preterm birth models induced by bacterial virulence factors.
Grant-in-Aid for Scientific Research (C)
|Allocation Type||Multi-year Fund |
|Research Institution||Research Institute, Osaka Medical Center for Maternal and Child Health |
YANAGIHARA Itaru 地方独立行政法人大阪府立病院機構大阪府立母子保健総合医療センター(研究所), 免疫部門, 部長 (60314415)
WU Heng Ning
MEHANDJIEV R Tzvetozar
|Project Period (FY)
2014-04-01 – 2017-03-31
|Keywords||ウレアプラズマ / LPS / 水酸化フラーレン / チオレドキシン１ / 感染性流早産|
|Outline of Final Research Achievements
Intrauterine infection such as by Escherichia coli and Ureaplasma spp. induce placental inflammation and are one of the leading causes of preterm birth. We investigated the preventative effect of hydroxylated fullerene in a mouse preterm birth model induced by ureaplasmal TLR-2 ligand, UPM-1. The preterm birth rate decreased from 72.7% to 18.2% after an injection with hydroxylated fullerene in mice.
The preventative effect of thioredoxin-1 was also investigated in Escherichia coli lipopolysaccharide induced preterm birth mouse model. Recombinant human thoredoxin-1 prevented the rise of systemic proinflammatory cytokine levels in dam mice. After LPS challenge, placentas exhibited severe maternal vascular dilatation and congestion and a marked decidual neutrophil activation. These placental pathological findings were ameliorated by recombinant human thioredoxin-1, and the rate of inflammation-induced preterm delivery was attenuated.
|Free Research Field