Analysis of autoimmune responses against DNA mismatch repair enzymes in dermatomyositis

2017 Fiscal Year Final Research Report

• Project/Area Number: 26461656
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Dermatology
• Research Institution: Nagoya University
• Principal Investigator: Muro Yoshinao 名古屋大学, 医学系研究科, 准教授 (80270990)
• Research Collaborator: MOMOHARA MARIKO ; YAMAMOTO ERI
• Project Period (FY): 2014-04-01 – 2018-03-31
• Keywords: 皮膚筋炎 / 自己抗体 / DNAミスマッチ修復酵素

Outline of Final Research Achievements

DNA mismatch repair is an important life system, which is conserved from bacteria to human, and starts by mismatch recognition where seven kinds of enzymes are involved in human. We reported that patients with collagen diseases including inflammatory myopathy carry autoantibodies against four kinds of these enzymes. Further studies clarified that major targets of these antibodies are MLH1 and PMS1 and that some patients who had these antibodies also simultaneously carried other myositis-marker autoantibodies and other patients did not. We have found that commercially available substrates for indirect immunofluorescence tests were not suitable for the screening of autoantibodies against these enzymes and the suitable fixed cultured cells for the screening. Moreover, we have found the possibility that ultraviolet irradiation to cultured keratinocytes might influence the expression of these enzymes (autoantigens).

Free Research Field

膠原病学