Lineage analysis of human epidermal keratinocytes and their actin filaments during stratification

2016 Fiscal Year Final Research Report

• Project/Area Number: 26461660
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Dermatology
• Research Institution: Tokyo Medical and Dental University (2015-2016); Ehime University (2014)
• Principal Investigator: NANBA Daisuke 東京医科歯科大学, 難治疾患研究所, 准教授 (10380255)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 表皮角化細胞

Outline of Final Research Achievements

The epidermis is a stratified squamous epithelium that functions as a bidirectional barrier. However, this barrier is maintained with dynamic behavior of keratinocytes. The epidermal keratinocyte stem cells contribute to epidermal homeostasis by supplying keratinocytes, and yet it is still unclear how the stem cells maintain this straitifed epithelial tissue. In this study, we have investigated the actin cytoskeleton in human keratinocyte stem cells, because actin cytoskeletal system regulates cellular behavior. First, we have established the imaging system of actin filaments in living human keeartinocytes with lifeact-venus probe. This probe elucidated the dynamics of actin filaments in human keratinocytes, and we found that the remodeling of actin filaments is involved in the migration and stratification of keratinocytes. We also found that actin cytoskeleton is involved in the stem cell maintenace.

Free Research Field

幹細胞生物学