2016 Fiscal Year Annual Research Report
Differential Day & Night outcome to cutaneous viral infection
| Project/Area Number |
26461685
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| Research Institution | University of Yamanashi |
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Principal Investigator |
川村 龍吉 山梨大学, 総合研究部, 准教授 (70262657)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | Herpes simplex virus / HSV receptor Nectin1 / circadian variations / clock genes |
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| Outline of Annual Research Achievements |
Herpes simplex virus type 2 (HSV-2) is a sexually transmitted pathogen that is widely prevalent around the world. To date, it is not fully understood whether the time of infection is a variable influencing the outcome of HSV-2 pathology. To address the issues,wild-type mice housed under 12-hour light/12-hour dark conditions were intradermally infected with HSV-2 either at ZT6 or at ZT18 (active phase). We found that the clinical severity and mortality were higher in mice infected at active phase than at rest phase, depending on circadian expression of HSV-2 receptor Nectin1 in the skin that is likely controlled by the molecular clock. In addition, the degree of the host innate immune response was also less severe in the skin of mice infected at ZT6 than at ZT18. Consistent with these findings, larger doses of acyclovir were required in mice infected at active phase to obtain comparable clinical severity and mortality to those of mice infected at rest phase.These findings provide new insight into previously unknown aspects of HSV-2 infection.
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