Identification andcharacterization of skin cancaer stem cells

2016 Fiscal Year Final Research Report

• Project/Area Number: 26461694
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Dermatology
• Research Institution: The University of Tokushima
• Principal Investigator: MURAO Kazutoshi 徳島大学, 大学院医歯薬学研究部(医学系), 准教授 (40363171)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 皮膚癌 / 有棘細胞癌

Outline of Final Research Achievements

There was a distinct population called side population cells about 1 to 2 % of human SCC cells, which was almost identical results with chemically induced mouse SCC. To determine the capacity of tumorigenesis of SP cells, I sorted 640 SP cells and non-SP cells, and tried to compare their tumorigenecity by in vitro assay, but non worked well. Next, I investigated the expression of CD98 in human SCC. About two third of SCC cells expressed CD98. To determine the relationship between SP cells and CD98 expression, I performed dual staining of Hoechst dye and CD98. Most of SP cells expressed CD98. I could not detect any SP cells in melanoma cell line or dermatofibrosarcoma protuberans cells.

Free Research Field

皮膚科学