2016 Fiscal Year Final Research Report
Melanocyte and iPS cell research for treatment of vitiligo and melanoma
| Project/Area Number |
26461704
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Dermatology
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| Research Institution | St. Marianna University School of Medicine |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | メラノサイト / 皮膚色素細胞 / iPS細胞 / 白斑 / 悪性黒色腫 / 白髪 |
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| Outline of Final Research Achievements |
We established a new in vitro approach to generate induced pluripotent stem (iPS) cell-derived human melanocytes which have higher proliferation rates and increased melanin production compared to melanocytes prepared by previously reported approaches. We designed two original methods, transferring black colonies by pipet and recovering black cell pellets from centrifuged medium, and numerous human iPS cell-derived melanocytes proliferated in gelatinous dishes coated with Matrigel after 12 days. We also succeeded in inducing melanin pigmentation in nude mouse skin in vivo using those human iPS cell-derived melanocytes. We propose that this new method using iPS cells established from T cells in the blood of normal human volunteers could be applied clinically to develop regenerative medicine and cell therapies for various forms of human pigmentation disorders.
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| Free Research Field |
皮膚科
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