Basic Study to evaluate DNA Damage and Cell Survival with Alpha-Particles from Astatine-211

2016 Fiscal Year Final Research Report

• Project/Area Number: 26461866
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Radiation science
• Research Institution: Gunma Institute of Public Health and Environmental Sciences
• Principal Investigator: WATANABE Naoyuki 群馬県衛生環境研究所, 研究企画係, 研究員 (90311381)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: がん治療 / RI内用療法 / α放出核種 / At-211 / 核医学治療

Outline of Final Research Achievements

The Purpose was to produce Astatine (At)-211 from a cyclotron and to investigate DNA damage and cell survival of cultured cancer cells with alpha-particles from At-211 for one of potential radionuclide treatments. At-211 was produced by a cyclotron via the Bi-209 (α, 2n) At-211 reaction. SKOV3 ovarian carcinoma cells were extracellularly irradiated with various radioactivity of At-211. Induction of Double-Strand Breaks (DSB) was then determined using single cell gel electrophoresis assay (Comet assay) and cell survivals were obtained by means of colony formation assay. In the cells, DSB were significantly observed, and a dose dependent of At-211 was shown. The cell survival rates steeply decreased with increasing radioactivity of At-211. In conclusion, At-211 was successfully produced by a cyclotron and separated. The cellular damage with α-particles from extracellular At-211 was observed with a dose dependent of the radionuclide.

Free Research Field

がん治療