The effect of ionizing radiation on secretory phenotype of cancer-associated fibroblasts and its impact on cancer pathophysiology

2016 Fiscal Year Final Research Report

• Project/Area Number: 26461875
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Radiation science
• Research Institution: Hokkaido University
• Principal Investigator: Yamamori Tohru 北海道大学, 獣医学研究科, 准教授 (00512675)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 癌関連線維芽細胞 / 放射線 / サイトカイン / 活性酸素種 / 癌

Outline of Final Research Achievements

Recent evidence suggests that cancer-associated fibroblasts (CAFs), which are the most abundant cell type in cancer stroma, and CAF-derived secretory factors play important roles in various aspects of cancer pathophysiology. This study was aimed to understand how ionizing radiation influences on CAFs and their secretory phenotype. We revealed that X-irradiation to mouse embryonic fibroblasts (MEFs) increased the number of senescent cells, which are reported to have similar characteristics to CAFs. This event was accompanied by the induction of multiple cytokines. We also found that X-irradiation enhanced cellular oxidative stress level and NADPH oxidase 4 protein (NOX4) was in part responsible for it. Furthermore, our data suggested that NOX4 was not involved in the induction of secretory factors in irradiated MEFs.

Free Research Field

放射線生物学