Analysis of micro RNA as new treatment target of radiation therapy for esophageal cancer on In vivo and clinical cases.

2017 Fiscal Year Final Research Report

• Project/Area Number: 26461893
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Radiation science
• Research Institution: Kyushu University
• Principal Investigator: Hirakawa Masakazu 九州大学, 大学病院, 准教授 (20380454)
• Co-Investigator(Kenkyū-buntansha): 中村 和正 九州大学, 大学病院, 准教授 (20284507) ; 三森 功士 九州大学, 大学病院, 教授 (50322748) ; 寺嶋 広太郎 九州大学, 先端医療イノベーションセンター, 助教 (40627676)
• Project Period (FY): 2014-04-01 – 2018-03-31
• Keywords: 食道癌 / 放射線治療 / マイクロRNA / バイオマーカー

Outline of Final Research Achievements

We previously detected microRNA (miR)-203a as miR involved in radioresistance of esophageal cancer (ESC) cells (Grants-in-Aid for Scientific Research, 20380454).
Purpose of this study is to identify new radiosensitive biomarker and treatment target relating chemoradiation for ESC. The expression of miR-203a was significantly lower in TE1 (Radioresistant ESC cell line) and higher in TE9 (Radiosensitive ESC cell line). On colony formation assay, overexpression of miR-203 impaired resistance to radiation in TE1. MiR-203 was identified as the downregulated miR in radioresistant cell line compared to radiosensitive cell line. Overexpression of miR-203 increased radiosensitivity of ESC cell lines. Our findings indicate that miR-203 can be a target for overcoming the radioresistance of ESC. We also evaluated expression of LASP1 and BMl1 which are target gene of miR-203a, however, we could not obtain the clear result that LASP1 and BMl1 might be radiosensitive gene in ESC cell lines.

Free Research Field

放射線腫瘍学