The exploration of cytoprotective effect of dimethyl fumarate on human islet

2016 Fiscal Year Final Research Report

• Project/Area Number: 26461907
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: General surgery
• Research Institution: Shinshu University
• Principal Investigator: MASUDA Yuichi 信州大学, 医学部附属病院, 助教(診療) (60467149)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 膵島 / 移植 / Nrf2経路 / 酸化ストレス

Outline of Final Research Achievements

The islets are known to vulnerable to oxidative stress because of containing less quantity of anti-oxidant enzymes. Oxidative stress will be given to islets during the process of freeze and thaw. Nrf2 is a key transcriptional factor to regulate the anti-oxidant genes expression. Dimethyl fumarate (DMF) is known as one of Nrf2 activators. The aim of this work is to investigate the cryoprotective effect of dimethyl fumarate (DMF) on rat and human islets.
On rat and human thawed islets treated with DMF, anti-oxidant enzyme mRNA expressions were significantly higher, and the percentage of 7-aminoactinomycin D positive cells were lower than those without DMF. On rat islet transplant to nude mouse model, the levels of blood glucose were significantly lower in DMF group(n=4 in each). On human islets transplanted to nude mice model, the average of the blood glucose in DMF group was low (n=2 in each).

Free Research Field

移植