Label-free imaging identification of cells using quantitative phase microscopy for negative selection of Circulating Tumor Cells

2016 Fiscal Year Final Research Report

• Project/Area Number: 26461974
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Digestive surgery
• Research Institution: Hamamatsu University School of Medicine
• Principal Investigator: Kawabata Toshiki 浜松医科大学, 医学部附属病院, 診療助教 (90402289)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 血中循環腫瘍細胞

Outline of Final Research Achievements

We observed WBCs and five cell line cells flowing in the chamber by quantitative phase microscopy (QPM) that provides quantitative morphological information with high contrast without staining. Then, we extracted certain features from the obtained images as training data for pattern recognition, and created an algorithm to differentiate WBCs from cell lines. The obtained algorithm successfully differentiated WBCs from cell lines (AUC=0.99). Our label-free, non-cytotoxic cell recognition method based on the features of QPM images has the potential to serve as a novel method for cell recognition and negative selection of circulating tumor cells (CTCs).

Free Research Field

消化器外科