2016 Fiscal Year Final Research Report
Detection of circulating tumor DNA in cell-free DNA in gastric cancer patients and imatinib-resistant GIST patients using next-generation sequencer
| Project/Area Number |
26461977
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | Osaka University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | ctDNA / 次世代シーケンサー / 胃癌 / GIST |
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| Outline of Final Research Achievements |
We investigated whether tumor-specific mutations could be detected in circulating tumor DNA (ctDNA) from the peripheral blood in patients with gastric cancer or imatinib-resistant GIST.
Cell-free DNA was extracted in blood samples in 6 patients with gastric cancer. Identical TP 53 mutations could be detected in ctDNA in 3 of the 6 patients. All 3 patients had extremely progressive disease, and the fraction rates of ctDNA were also consistent with tumor volume and disease course. Regarding GIST, imatinib-resistant lesions had single nucleotide substitutions in C-KIT exon 13 in three patients and exon 18 in one patient. Identical secondary C-KIT mutations could be detected in ctDNA with a mutant fraction range of 0.010- 9.385%, and the fraction rate of ctDNA was consistent with tumor volume and disease course.
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| Free Research Field |
医歯薬学
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