Association of lymphatic endothelial cells with immuno tolerance and identification of predictive marker of metastasis in gastric cancer

2016 Fiscal Year Final Research Report

• Project/Area Number: 26461990
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Digestive surgery
• Research Institution: Osaka City University
• Principal Investigator: Tanaka Hiroaki 大阪市立大学, 大学院医学研究科, 講師 (90382168)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 胃癌 / リンパ節転移

Outline of Final Research Achievements

We aimed to determine whether tumor-specific LECs inhabit the tumor microenvironment and examine their influence on this microenvironment. We isolated normal LECs (NLECs) from a non-metastatic lymph node and tumor-associated LECs (TLECs) from cancerous lymph nodes.When compared to NLEC, TLECs had an abnormal shape, high proliferative and migratory abilities, and elevated expression of genes associated with inflammation, cell growth, and cell migration. NLECs co-cultured with gastric cancer cells from the OCUM12 cell line acquired TLEC-like phenotypes. Also, OCUM12 cells co-cultured with TLECs expressed high levels of genes responsible for metastasis. Our results demonstrated that LECs interacted with tumor cells and obtained abnormal phenotypes that could play important roles in tumor progression

Free Research Field

消化器外科