Development of therapy for cancer stem cell targeting miRNA in esophageal squamous cell carcinoma

2016 Fiscal Year Final Research Report

• Project/Area Number: 26461996
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Digestive surgery
• Research Institution: Keio University
• Principal Investigator: Fukuda Kazumasa 慶應義塾大学, 医学部(信濃町), 特任講師 (50348786)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: miRNA / 食道癌

Outline of Final Research Achievements

In the present study, we investigated miRNA expression profile and function in CSC-like clones and serum derived from esophageal squamous cell carcinoma (ESCC) patients. The result exhibited common 67 miRNAs differentially expressed between CSC-like clones and their parent cell lines. The genes predicted to be targets of candidate miRNAs were chosen and analyzed using the database of miRNA.org site. miR-125a-5p has important roles in docetaxel resistance by regulating protein expression of the ICIS gene. Analysis of comprehensive expression of miRNA in serum was performed using the 3D-Gene system. Between lymph node metastasis group and non-metastasis group, 33 miRNAs significantly showed expression fluctuation. These findings suggest that it may be helpful to develop novel strategies for targeted therapies in ESCC patients.

Free Research Field

分子生物学