Clinical significance of the exosome detected in the pancreatic juice in the setting of preoperative chemoradiation therapy for pancreatic adenocarcinoma

2016 Fiscal Year Final Research Report

• Project/Area Number: 26462079
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Digestive surgery
• Research Institution: Research Institute, Osaka Medical Center for Cancer and Cardiovascular Disaeses
• Principal Investigator: Takahashi Hidenori 地方独立行政法人大阪府立病院機構大阪府立成人病センター(研究所), その他部局等, 副部長 (90601120)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 膵癌 / エクソソーム / 術前化学放射線療法 / 効果予測

Outline of Final Research Achievements

The aim of this study is to investigate the clinical significance of the exosome obtained in the pancreatic juice in the setting of preoperative chemoradiation therapy for pancreatic cancer (PC). First, we revealed that the pancreatic juice contains the exosome suitable quality for the analysis of mRNA and miRNA expression and that those exosome could be extracted from the pancreatic juice obtained during ERCP under various conditions. Second, we extracted the exosomes from the serum obtained from the patient with PC who were treated by preoperative chemoradiation therapy, and micro RNAs in those exosome were assessed using the micro RNA array. We identified several miRNAs (including mir-22，mir-122,and mir-320) that were significantly associated with the prognosis. Further investigation is planned to assess the biological significance of those miRNAs obtained from the pancreatic juice with the special attention to predict the therapeutic effect of preoperative treatment for PC.

Free Research Field

医学