2016 Fiscal Year Final Research Report
The Development of Cryo-Vetrification Method in Human Myoblast Cell Sheet for Propagation of Regeneration Therapy
| Project/Area Number |
26462089
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Cardiovascular surgery
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| Research Institution | Osaka University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | 細胞シート / ガラス化凍結 / 骨格筋芽細胞 / iPS細胞由来心筋細胞 |
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| Outline of Final Research Achievements |
We explored the clinically applicable cryopreservation method of skeletal myoblast sheet and iPS cells-derived cardiomyocytes (CMs) sheet. As a result, the vitrified frozen human skeletal myoblast sheet maintained the functionality and structure of cell-sheet. In addition, transplantation of the thawed human myoblast sheet from the vitrified cryopreservation induced the improvement of cardiac function in the myocardial infarction (MI) nude rat.
In the iPS cell-derived CMs, the purity of CMs did not change by the vitrified frozen and the thawing process. The thawed CMs sheet showed spontaneous synchronized beatings. To check the impacts on cardiac performance in vivo, we transplanted the cell sheets of non-cryopreserved CMs and cryopreserved CMs into nude rats with MI. There was no significant difference in the efficiency between the non-cryopreserved and cryopreserved groups, indicating that the efficiency of cryopreserved CMs in vivo was comparable to that of freshly prepared CMs.
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| Free Research Field |
再生医療
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