Efficacy and mechanism of Multilineage-differenciating Stress Enduring Cell transplantation in experimental abdominal aortic aneurysm

2016 Fiscal Year Final Research Report

• Project/Area Number: 26462097
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Cardiovascular surgery
• Research Institution: Tohoku University
• Principal Investigator: TAKAHASHI GORO 東北大学, 医学系研究科, 非常勤講師 (50526449)
• Research Collaborator: Katsuhiro Hosoyama 東北大学, 医学系研究科, 大学院生
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 大血管外科学 / 大動脈瘤 / Muse細胞 / 再生医療

Outline of Final Research Achievements

Cell therapies for aortic aneurysms (AAs) have advantages of non-invasiveness compared to surgical therapies. However previously reported cell therapies had only limited therapeutic effect. With the aim of evaluation the efficacy of multi-lineage differentiating stress enduring cells (Muse cells), which are a kind of pluripotent stem cells with unique features, abdominal aortic aneurysms were induced in mice models and assessed the AAs diameter after cell transplantation. Muse cells were collected from human bone marrow mesenchymal stem cells by FACS sorting with SSEA-3 staining. The mean diameters of AAs in Muse group were significantly smaller than other groups. The immunofluorescent assessment demonstrated that some smooth muscle cells and endothelial cells were derived from injected Muse cells. These data suggest that Muse cells can fix AAs by regenerating the damaged cell components of aortic wall.

Free Research Field

心臓血管外科分野