Novel approach to acute limb ischemia with nano-DDS

2016 Fiscal Year Final Research Report

• Project/Area Number: 26462100
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Cardiovascular surgery
• Research Institution: The University of Tokyo
• Principal Investigator: Nishiyama Ayako 東京大学, 医学部附属病院, 登録研究員 (90705526)
• Co-Investigator(Renkei-kenkyūsha): KUROHMARU Masamichi 東京大学, 大学院農学生命科学研究科, 教授 (00148636)
• Research Collaborator: SUHARA Masamitsu 東京大学, 医学部附属病院, 病院診療医
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 急性下肢虚血 / 血管新生療法 / ドラッグデリバリー / ナノメディシン

Outline of Final Research Achievements

We made an acute hindlimb ischemia model by resecting left femoral artery. In this model, collateral arteries (naturally-made bypass blood flow) developed mainly in two kinds of thigh muscles, called semi-membranous and caudofemoralis muscle. We injected fluorescent-labeled hollow nano-size particle, called "PICsome" into this model intravenously. PICsomes accumulated to the same muscles as described above. We also administered another nano-particle called "PIC micelle" which include the agent to promote the angiogenesis. However, there was no similar accumulation to the muscles. Because PIC micelle has less stability in the living body, we suppose that they dissolved spontaneously before accumulating to the target muscles. In future, we are planning to achieve accumulation and therapeutic improvement by enhancing the stability of PIC micelle or increasing the dosage. Moreover, we expect that this study can expand to other fields such as diagnostic imaging device.

Free Research Field

血管外科