Application of cell sheets for therapy of refractory ulcers in ischemic hindlimbs

2016 Fiscal Year Final Research Report

• Project/Area Number: 26462109
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Cardiovascular surgery
• Research Institution: Yamaguchi University
• Principal Investigator: MURAKAMI Masanori 山口大学, 医学部, 特別医学研究員 (30448295)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: ヒト細胞混合シート / ヒト末梢血単核球 / ヒト線維芽細胞 / VEGF

Outline of Final Research Achievements

We performed functional analyses of mixed human cell sheets consisting of human peripheral blood mononuclear cells and human fibroblasts. The amount of vascular endothelial growth factor (VEGF) secreted from mixed human cell sheets was 2-fold higher than that from human fibroblasts. Furthermore, the amount of vascular endothelial growth factor (VEGF) secreted from mixed human cell sheets cultured under hypoxia (2% oxygen) was 2-fold higher than that from mixed human cell sheets cultured under normoxia (20% oxygen). These results revealed that mixture of human peripheral blood mononuclear cells with human fibroblasts and cell culture of mixed human cell sheets under hypoxia lead to enhanced secretion of VEGF.

Free Research Field

心臓血管外科